Plaque hybridization
Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages.[1] The procedure can also be used for the detection of differentially represented repetitive DNA. The technique (similar to colony hybridization) involves hybridizing isolated phage DNA to a label probe for the gene of study. This is followed by autoradiography to detect the position of the label.[2] The plaque hybridization procedure has some advantages over colony hybridization due to the smaller and well defined area of the filter to which the DNA binds.[3]
References[edit]
- ^ Dale, J. W.; Greenaway, P. J. (1985). Nucleic Acids. Methods in Molecular Biology. Vol. 2. pp. 285–8. doi:10.1385/0-89603-064-4:285. ISBN 0-89603-064-4. PMID 21374207.
- ^ Meister, G. A.; Lansman, R. A.; Grigliatti, T. A. (1995). "Simple plaque hybridization method for the detection of differentially represented repetitive DNA". BioTechniques. 18 (2): 250–5. PMID 7727126.
- ^ "Nylon Membranes for Colony and Plaque Hybridization". Life science. Retrieved June 4, 2015.