Talk:Hybridoma technology

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Question[edit]

The article isn't clear. THere's no enough data on what a hybridoma is (does it multiply, for example? what immortal actually means? it multiplies forever, or a specific cell doesn't die?, the cell has 43 chromosomes? if so how it multiplies?) Gabrieloncho (talk) 13:59, 5 April 2010 (UTC)[reply]

I agree. --David Tornheim (talk) 01:20, 14 February 2016 (UTC)[reply]

Discovery of hybridomas[edit]

Will someone please add in some history on the discovery of hybridomas? Here's an article: http://www.sciencemag.org/cgi/reprint/215/4536/1073 128.12.37.211 (talk) 11:55, 13 May 2008 (UTC)हाइब्रिडोमा टेक्नोलॉजी बड़ी संख्या में समान एंटीबॉडी (जिसे मोनोक्लोनल एंटीबॉडी भी कहा जाता है) बनाने का एक तरीका है। यह प्रक्रिया एक एंटीजन के साथ एक माउस (या अन्य स्तनपायी) इंजेक्शन लगाने से शुरू होती है जो एक प्रतिरक्षा प्रतिक्रिया को उत्तेजित करता है एक प्रकार का सफेद रक्त कोशिका, बी सेल जो एंटीबॉडी पैदा करता है जो एंटीजन को बांधता है तब माउस से काटा जाता है। इन अलग बी कोशिकाओं बारी में अमर बी सेल कैंसर की कोशिकाओं को, एक मायलोमा, साथ जुड़े हुए हैं एक संकर सेल लाइन एक हाइब्रिडोमा कहा जाता है, जो बी कोशिका के दोनों प्रतिरक्षी-उत्पादक क्षमता और अतिरंजित लंबी उम्र और मायलोमा की प्रजनन क्षमता है निर्माण करने के लिए । हाइब्रिडोमास संस्कृति में उगाया जा सकता है, प्रत्येक संस्कृति एक व्यवहार्य हाइब्रिडोमा सेल के साथ शुरू, संस्कृतियों, जिनमें से प्रत्येक आनुवंशिक रूप से समान हाइब्रिडोमास जो संस्कृति प्रति एक एंटीबॉडी (मोनोक्लोनल) के बजाय अलग एंटीबॉडी (पॉलीक्लोनल) के मिश्रण से उत्पादन के होते हैं का निर्माण किया। इस प्रक्रिया में उपयोग की जाने वाली मायलोमा सेल लाइन को टिशू कल्चर में बढ़ने की क्षमता और एंटीबॉडी संश्लेषण की अनुपस्थिति के लिए चुना गया है। पॉलीक्लोनल एंटीबॉडी, जो कई अलग अलग एंटीबॉडी अणुओं के मिश्रण हैं के विपरीत, मोनोक्लोनल प्रत्येक हाइब्रिडोमा लाइन द्वारा उत्पादित एंटीबॉडी सभी रासायनिक समान हैं।[reply]

मोनोक्लोनल एंटीबॉडी के उत्पादन सीज़र मिल्स्तीं और जार्ज जे एफ कोहलर द्वारा आविष्कार किया गया था 1975 में वे नील्स काज जर्न, जो प्रतिरक्षा विज्ञान के लिए अन्य योगदान दिया साथ चिकित्सा और फिजियोलॉजी के लिए 1984 के नोबेल पुरस्कार साझा की है। 1 976-19 77 में सेसर मिल्स्टीन की प्रयोगशाला में अपने विश्राम के दौरान लियोनार्ड हर्जेनबर्ग द्वारा शब्द हाइब्रिडोमा को गढ़ा गया था। [1]

Merged partly diverging descriptions of process[edit]

Text I removed because it contradicts the rest of the description, or because it is unclear:

While some may believe that IL-6 is essential for this step, it is not necessary to add that expensive supplement, rather use 50% heat-inactivated FBS for the first week.^{[citation needed]} Add 10% FBS DMEM to the clone culture plate after screening for single colony wells.

After using HAT it is often desirable to use HT containing media.

--ἀνυπόδητος (talk) 12:39, 5 January 2010 (UTC)[reply]

Aug 2014 Edit[edit]

You will see my note in my Aug 2014 edit. I recommend that relevent content is moved to the mAb page. Also, some text was removed, but captured in higher level of detail in summary (e.g. more specific / lower threshold than staining) and the text regarding the benefit of diagnostics using mAb to avoid unnecessary procedures.

I recommend this page be evaluated by an expert and discuss 'Hybridoma technology' only. January2009 (talk) 14:40, 18 August 2014 (UTC)[reply]

Making this article more accessible[edit]

@Boghog: Thank you! Huge improvement. So is one of the main purposes of creating these anti-bodies (besides research) to make vaccines or as a drug to fight off infection of some sort? I guess I don't really understand why one would want to mass produce anti-bodies besides research. I know the human body makes anti-bodies to kill off colds and/or viruses and perhaps other pathogens, but I thought Penicillin was the only drug (a fungus I believe) that could actually kill a cold (bacteria), and that there was pretty much no medical cure for any cold stemming from a virus. So I am a little confused about how these manufactured anti-bodies can be used. --David Tornheim (talk) 11:20, 14 February 2016 (UTC)[reply]

Thanks. This is somewhat outside my area of expertise, so I hope I haven't introduced any errors. Monoclonal antibodies produced by hybridomas are used for all sorts of things. In addition to the most obvious application of helping the immune system to fight off infection, they can engineered to block the immune system in inflammatory diseases (see biopharmaceutical). Antibodies are also used in ELISA as diagnostic tools. The current article focuses on diagnostics but this article probably should be expanded to include more on the development and manufacture of therapeutics. Boghog (talk) 13:10, 14 February 2016 (UTC)[reply]