Small t intron
 | This article provides insufficient context for those unfamiliar with the subject. (October 2009) |
Plasmid vectors are circular strands of DNA, found in virions, that are used in genetic engineering to integrate new genes into a host cell genome.
The small T intron is an intron, that is used in some plasmid vectors, in order to induce gene expression in mammalian cells.
Function[edit]
The function of this intron in the vectors is unknown, but it is theorized that it might be involved in splicing or translation efficiency.[1][2]
Vectors such as pME18s contain it.
References[edit]
- ^ Huang MT, Gorman CM (1990). "The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing". Mol Cell Biol. 10 (4): 1805–10. doi:10.1128/mcb.10.4.1805. PMC 362290. PMID 1690852.
- ^ Hunt AG, Mogen BD, Chu NM, Chua NH (1991). "The SV40 small t intron is accurately and efficiently spliced in tobacco cells". Plant Mol Biol. 16 (3): 375–9. doi:10.1007/bf00023989. PMID 1654158. S2CID 9326989.
 | This cell biology article is a stub. You can help Wikipedia by expanding it. |