REC8

REC8
Identifiers
Aliases	REC8, HR21spB, REC8L1, Rec8p, REC8 meiotic recombination protein
External IDs	OMIM: 608193 MGI: 1929645 HomoloGene: 3768 GeneCards: REC8
Gene location (Human) Chr. Chromosome 14 (human) Band 14q12 Start 24,171,853 bp End 24,180,257 bp
Gene location (Mouse) Chr. Chromosome 14 (mouse) Band 14 C3|14 28.19 cM Start 55,855,494 bp End 55,862,852 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in anterior pituitary ganglionic eminence right uterine tube bone marrow gallbladder body of stomach bone marrow cells spleen fundus hypothalamus Top expressed in spermatid seminiferous tubule duodenum urethra islet of Langerhans seminal vesicula morula adrenal gland Paneth cell jejunum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function chromatin binding Cellular component male germ cell nucleus chromosome lateral element condensed nuclear chromosome nuclear chromosome synaptonemal complex chromosome, centromeric region nucleus condensed chromosome meiotic cohesin complex cohesin complex Biological process reciprocal meiotic recombination chromosome segregation male meiosis I sister chromatid cohesion seminiferous tubule development homologous chromosome pairing at meiosis oocyte maturation double-strand break repair via homologous recombination synaptonemal complex assembly spermatogenesis spermatid development fertilization double-strand break repair meiosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9985 56739 Ensembl ENSG00000100918 ENSMUSG00000002324 UniProt O95072 Q8C5S7 RefSeq (mRNA) NM_001048205 NM_005132 NM_020002 NM_001360389 NM_001360390 RefSeq (protein) NP_001041670 NP_005123 NP_064386 NP_001347318 NP_001347319 Location (UCSC) Chr 14: 24.17 – 24.18 Mb Chr 14: 55.86 – 55.86 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Meiotic recombination protein REC8 homolog is a protein that in humans is encoded by the REC8 gene.

Rec8 is a meiosis-specific component of the cohesin complex that binds sister chromatids in preparation for the two divisions of meiosis. Rec8 is sequentially removed from sister chromatids. It is removed from the arms of chromosomes in the first division - separating homologous chromosomes from each other. However, Rec8 is maintained at centromeres so that sister chromatids are kept joined until anaphase of meiosis II, at which point removal of remaining cohesin leads to the separation of sister chromatids.

Function

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. REC8 protein appears to participate with other cohesins STAG3, SMC1B and SMC3 in sister chromatid cohesion throughout the whole meiotic process in human oocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Rec8 remains in complex with SMC proteins until anaphase, where it is degraded by Separase once the spindle assembly checkpoint is bypassed. Unlike the other Kleisin family member, Scc1, Rec8 must be phosphorylated prior to degradation. Prior to anaphase, Rec8 is protected from phosphorylation by Protein Phosphatase 2 (PP2A-B56) in mouse. PP2A is recruited to cohesin by Shugoshin 2 (Sgo2; SGOL2 in yeast). Bypass of the spindle assembly checkpoint activates Separase, which then degrades phosphorylated Rec8 and untethers Cohesin from sister chromatids, allowing for segregation of chromosomes.

Interactions

REC8 has been shown to interact with SMC3.

Further reading

• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Prieto I, Tease C, Pezzi N, Buesa JM, Ortega S, Kremer L, Martínez A, Martínez-A C, Hultén MA, Barbero JL (2004). "Cohesin component dynamics during meiotic prophase I in mammalian oocytes". Chromosome Research. 12 (3): 197–213. doi:10.1023/B:CHRO.0000021945.83198.0e. PMID 15125634. S2CID 411379.
• Schleiffer A, Kaitna S, Maurer-Stroh S, Glotzer M, Nasmyth K, Eisenhaber F (Mar 2003). "Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners". Molecular Cell. 11 (3): 571–5. doi:10.1016/S1097-2765(03)00108-4. PMID 12667442.