Gertrude married Vladislaus II, the eldest son of the late Duke Vladislaus I of Bohemia in 1140. Her husband had left the Duchy of Bohemia during the rule of his uncle, Duke Soběslav I, but was recalled by the local nobility after Soběslav's death. Through her mother, Gertrude was a half-sister of the Hohenstaufen king Conrad III of Germany, thus a good catch for Vladislaus. At the time of the siege of Prague by his cousin Count Conrad II of Znojmo in 1142, she successfully defended Prague Castle with the help of her brother-in-law Děpold, while Vladislaus sought assistance from King Conrad III.
Gertrude participated in projects of her husband Vladislaus to found new religious institutions and due to her encouragement, the duke invited foreign religious orders to establish themselves in Bohemia. She gave birth to five children and died in 1150 at the age of 30 at her residence in Prague.
