F-tractin

F-Tractin is a cellular probe for filamentous actin. It consists of a portion of the amino terminal actin binding region of the rat protein ITPKA, usually fused to a reporter such as green fluorescent protein. Initial studies determined that amino acids 9-52 from the rat ITPKA were useful as a live-cell reporter for actin filaments. Later studies determined that amino acids 9-40 were sufficient for F-actin binding.^[1]^[2]^[3] F-Tractin is one of a number of F-actin probes useful in live cell imaging.^[4]

References[edit]

^ Schell MJ, Erneux C, Irvine RF (2001). "Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus". J Biol Chem. 276 (40): 37537–46. doi:10.1074/jbc.M104101200. PMID 11468283.
^ Johnson HW, Schell MJ (2009). "Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology". Mol Biol Cell. 20 (24): 5166–80. doi:10.1091/mbc.E09-01-0083. PMC 2793293. PMID 19846664.
^ Yi J, Wu XS, Crites T, Hammer JA (2012). "Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells". Mol Biol Cell. 23 (5): 834–52. doi:10.1091/mbc.E11-08-0731. PMC 3290643. PMID 22219382.
^ Melak M, Plessner M, Grosse R (2017). "Actin visualization at a glance". J Cell Sci. 130 (3): 525–530. doi:10.1242/jcs.189068. PMID 28082420.

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[pmid11468283-1] Schell MJ, Erneux C, Irvine RF (2001). "Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus". J Biol Chem. 276 (40): 37537–46. doi:10.1074/jbc.M104101200. PMID 11468283.

[pmid19846664-2] Johnson HW, Schell MJ (2009). "Neuronal IP3 3-kinase is an F-actin-bundling protein: role in dendritic targeting and regulation of spine morphology". Mol Biol Cell. 20 (24): 5166–80. doi:10.1091/mbc.E09-01-0083. PMC 2793293. PMID 19846664.

[pmid22219382-3] Yi J, Wu XS, Crites T, Hammer JA (2012). "Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells". Mol Biol Cell. 23 (5): 834–52. doi:10.1091/mbc.E11-08-0731. PMC 3290643. PMID 22219382.

[pmid28082420-4] Melak M, Plessner M, Grosse R (2017). "Actin visualization at a glance". J Cell Sci. 130 (3): 525–530. doi:10.1242/jcs.189068. PMID 28082420.

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