File:Localization of a fluorophore using MINFLUX.png
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Summary
| Description |
English: MINFLUX localizes an active fluorescent molecule using a probing scheme. The doughnut-shaped excitation beam is positioned at different points at the periphery and in the middle of a probing area L. The active molecule is excited at each position, and photon fluxes are recorded. MINFLUX uses the flux patterns of these recordings to recenter the excitation beam closer to the active molecule and then performs another probing iteration. With each iteration, the probing area L is constricted, and the intensity zero of the excitation beam more accurately overlaps with the position of the active molecule. |
| Date | |
| Source | Own work |
| Author | AByolia |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 19:53, 29 January 2024 | | 3,672 × 2,118 (1.73 MB) | AByolia | Uploaded own work with UploadWizard |
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